AI-Generated example
Please Note: The content below is not a direct copy from the original Word document. It was generated by Gemini 3 Pro by combining information from multiple sections. It serves as a demonstration of how we can improve the SOPs using Docusaurus and Markdown formatting to make them more readable and accessible.
Microscopy & High-Throughput
Microscopy (General)
Controls
- Background: Strain with NO fluorophore.
- Bleedthrough: Strains with single tags (for co-localization).
- Oil Objective: Clean with 100% ethanol/isopropanol. Label plates used with oil.
- Brightfield: Always take a BF image for segmentation.
Eva Microscope Startup
Startup Sequence
- Main Power: Wait for start-up noises.
- RTC Controller: Wait for two beeps.
- Lasers: Wait for "Laser Ready" light.
- TPC: Turn on before opening software.
Troubleshooting Table
| Problem | Solution |
|---|---|
| Connectivity | Restart TPC. Check Tango box (blue light). |
| Focus | Clean plate/objective. Check Z-limits (Yeast: 6335µm). |
| CellSens Issues | Perform "ScanR-bypass": Close CellSens -> Open/Close ScanR -> Re-open CellSens. |
Singer & SGA
Singer Pads
- Put dirty pads IMMEDIATELY in bleach. Dried yeast acts like glue.
- Do not overfill washing boxes (pads should not float).
- Check pins before use—dispose of crooked pins.
- Ordering Plates: Order 20% more than needed to account for errors.
- SGA Spores: Keep SPO plates outside the fridge after replicating. Sporulation is inefficient; you may need to go back to them.
- Libraries: If picking from libraries, always streak to singles. Perform check PCR.
Library Cards
Use the "library cards" file in the Libraries folder to track missing colonies when refreshing.